Transgenic Facility
What we do
Injection of foreign DNA into occyte
The EMBL Transgenic Core Services in the Mouse Biology Unit at the Monterotondo Outstation offers biologists an affordable opportunity to expand their research into mouse models. ETCS provides the expertise and capacity to produce genetically modified cells and animals for EMBL members and the European scientific community.
ETCS Services
Injection of ES cells into blastocyst.
Transgenic mice production
Microinjection of foreign DNA or lentivirral vectors into fertilized oocytes of inbred or hybrid mouse strains.
- Transgenesis by DNA injection
- Transgenesis by BAC and YAC injection
- Transgenesis by Lentivirus injection
Conventional or conditional knock-out or knock-in mouse production
Microinjection or morula aggregation of genetically modified ES cells using blastocysts of different mouse strains.
- Electroporation of ES cells with targeting vector
- Screening of selected clones
- Blastocyst injection of targeted ES cells
- Production of chimeric embryos by morula aggregation
Rederivation services
Rederivationn by embryo transfer is the safest technique to obtain healthy mice from any contaminated mouse colony in order to introduce them in a clean or SPF facility.
Cryopreservation services
Cryopreservation is one of the most reliable techniques to maintain genetically modified mouse strains. Cryopreservation of two cell and eight cell embryos, morulas and sperm is available.